Formulation of various MOPS buffers in biochemical experiments

In biochemistry experiments, buffer solution formulation is the most important part of the experiment, and a truly reliable formula can change the fate of the experiment. MOPS, is suitable for the study of electron transport and phosphorylation of chloroplast thin layer samples. It can be prepared into a variety of Agar media and used as a nontoxic buffer in the limit medium for the cultivation of Streptomyces rodiculata and the production of cephalosporins, and can be used as the electrolyte system component of (IEF) in two-dimensional gel electrophoresis. It can also be used in Northern hybridization as a buffer for the separation and membrane transfer of RNA. What is the formula for such an important buffer? This paper summarizes almost All MOPS buffer formulations are for your reference.

(1) MOPS Buffer (10 ×)
Reagent mass (1L) final concentration
Ultra pure MOPS 41.86 g (or 46.26 g sodium salt) 0.2m
Anhydrous sodium acetate 4.1g (or 6.8g sodium acetate trihydrate) 0.05 M
Na2EDTA (0.5M, pH 7.5) 20 mL 0.01 M

Note:
Set pH to 7. 0 with NaOH (if MOPS sodium salt is used, adjust pH); with glacial acetic acid)
No need for high pressure sterilization;
Store without light at room temperature and cover with aluminum foil.

(2)MOPS buffer (5X)
Reagent mass (100 mL) final concentration (5X)
MgSO4 0.1204 g 10 mM
MOPS 10.463 g 0.5 M
NaCl 14.61 g 2.5 M
The reagents were dissolved in 50mL H2O, and pH was adjusted to 7.5 with NaOH, and the volume was 100 mL. Filter disinfection, avoid light storage.

(3)MOPS salts
Reagent MOPS Tricine FeSO4 7H2O NH4Cl K2SO4 CaCl2 2H2O MgCl2
NaCl
400mM 40mM 0.1mM 95mM 2.8mM 5 μ M 5.3mM 0.5m
The final volume of the solution prepared by the above reagent is 1 L, which is filtered and sterilized.

(4)MOPS-EGTA buffer
Final concentration of reagent mass (for 1L)
MOPS 20.9 g 0.1 M
MgSO4 0.24 g 2 mM
EGTA 0.38 g 1 mM
NaCl 29.2 g 0.5 M
The above reagents were dissolved in the water treated with 800mL DEPC, and pH was adjusted to 7.5 with NaOH. The buffer solution was filtered and sterilized by 0.22 μ m filter.

(5) MOPS electrophoresis buffer
0.2M MOPS (pH 7.0), 20mM sodium acetate, 10mM EDTA (pH 8.0).
10x solution: dissolve 41.8 g MOPS in 700mL DEPC treated water and adjust pH to 7.0 with 2N NaOH. The volume of 1 M sodium acetate treated with 20mL DEPC and 0.5 M EDTA (pH8.0) of 20Ml DEPC was fixed to 1 liter. Filter and sterilize, store at room temperature and avoid light.

(6)PFA-MOPS-EGTA fixation buffer
Reagent mass (50 mL) final concentration
Paraformaldehyde 2 g 4% (w / v)
NaOH (1 N) 5 mL /
HCl (1 N) 5 mL /
MOPS-EGTA 50 mL /
2 g paraformaldehyde was added to 1 N NaOH of 5mL and dissolved at 60 °C. 5mL 1N HCl and 35mL MOPS-EGTA buffer were added.

Hopefully these recipes will help you to succeed in the experiment.（http://www.hnhbsj.com）