Common problems with eBioscience flow antibodies

q. Can vortex shock antibody or recombinant protein?（http://www.hnhbsj.com）
We do not recommend concussion protein or antibody solution, which will change the characteristics of the product. Usually during transportation, the liquid may have been dispersed in the pipe. Recommended before use, instantaneous centrifugation, so that the antibody will sink to the bottom of the tube to achieve the labeled volume.

q. Can I scroll the cell samples that have been labeled with antibodies?
Yes, short term oscillations can keep the cells in the best shape.

q. Can cell samples labeled with tandem dyes, such as APC-eFluor? 780, PE-Cy7, be immobilized and preserved?
In most cases, samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μ L sample mixed with 100 μ L IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. In 00-5333), 4 °C in the dark can be preserved for up to 3 days without any effect on detection. Some of the effects may be related to clone numbers.

q. How can I keep samples that can't be tested immediately?（http://www.hnhbsj.com）
First of all, to obtain the best results, must be recommended for immediate computer testing.
To save the dyed samples, you can use the IC Fixation Buffer (cat. 00-8222) (100 μ L sample mixed with 100 μ L IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. In 00-5333), it can be kept for up to 3 days at 4 °C in the dark. For tandem dyes such as APC-eFluor? 780, PE-Cy7, etc., eBioscience Data display does not increase their compensation with APC or PE. Some of the effects may be related to clone numbers.

q. Can I use my own fixed buffer to preserve cells?
We do not recommend the use of other buffers, we can only make sure that IC Fixation Buffer (cat. 00-8222) or 1-step Fix/Lyse Solution (cat. 00-5333).

q. There are references to incompatibility with DEC205 cells. What other markers can I choose?
In fact, CD205 has been proved to be able to bind to PE-Cy5.5 labeled antibody and streptavidin labeled (Part et al.. 2012 J. Immunol Methods 384: 184-90); however, it cannot be used in CD205 dendritic cells. We recommend using PerCP-Cy5.5 or PerCP-eFluor 710 tags instead.

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For more information on biochemical reagents, check out Huibai Reagent's official website: http://www.hnhbsj.com
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