YNB medium formula and sterilization method

YNB medium, also known as amino-free yeast nitrogen medium, the following is TNB medium formula, this product is used only for research and development, not for medicine, family or other uses. Other ingredients can be properly added or reduced according to the culture.

YNB medium formula
Nitrogen Source (nitrogen source)
Ammonium Sulfate (ammonium sulfate) 5g
Vitamins (vitamins)
Biotin (Biotin)2. mu.g
Calcium Pantothenate (calcium pantothenate) 400 μ g
Folic Acid 2.0. mu.g
Inositol (Inositol)2, 000.0. mu.g
Niacin (niacin)400 & mu; g
P - Aminobenzoic Acid (p-aminobenzoic acid) 200 μg.
Pyrioxine Hydrochlorin (Hydroxanol, Hydrochloric Acid)400 & mu; g
Ribofungin (riboflavin) 200.mu. g
chloramine hydrochloride (thiamine hydrochloride)400 & mu; g
Compounds Supply Trace Elements
Boric Acid (Boric Acid)500 & mu; g
Copper Sulfate (Cupric Sulfate)40 & mu; g
Potassium Iodide (potassium iodide) 100 μ g
Ferric Chloride (Ferric Chloride)200 & mu; g
Mangese Sulfonate (Manganese Sulfate)400 & mu; g
Sodium Molybdate (sodium molybdate) 200 μ g
Zinc Sulfate (zinc sulfate) 400 μ g
Salts (salt)
Monopotassium Phosphate (potassium dihydrogen phosphate) 1g
Magnesium Sulfate (magnesium sulfate 0.5g
Sodium Chloride (Sodium Chloride) 0.1g
Calcidium Chloride (Calcium Chloride) 0.1g

Sterilization method of YNB medium
Filtration and sterilization: some plant growth regulators and organic compounds, such as IAA,GA3,ZT,CM, are easy to decompose in the event of heat and can not be sterilized at high temperature with the culture medium, but use bacterial filters to filter out the miscellaneous bacteria. Bacterial filter and filter membrane (pore size 0.45 μ m) should be sterilized by high pressure before using. The filtered solution should be added to the medium immediately, if it is a liquid medium, it can be added when the medium is cooled to 30 ℃, and if it is a solid medium, it must be added before the solidification of the medium (50 × 60 ℃) to make the solution mix well with other components.

The above is YBN medium formula, YNB medium for some microorganisms and microorganisms for special purposes, but also pay attention to gas factors and light stimulation.

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