Do you know what kind of Congo red medium you belong to?

Culture medium is an artificial nourishment for the growth and maintenance of microorganisms, plant tissues and animal tissues. Generally divided into natural medium, combination medium, solid medium, liquid medium and so on, so what kind of Congo red medium we commonly use?

What kind of medium does the Congo red medium belong to:

The Congo red medium belongs to the solid medium, because the transparent circle formed by the decomposition of cellulose can be seen on the medium, but it is impossible to form the transparent circle in the liquid medium.

It is an identification medium because other microorganisms can also grow on the medium, such as some autotrophic microorganisms can survive on the medium; however, only the cellulose decomposing bacteria around will form a transparent circle, thus identifying the colony.

It is also the case where the culture medium is not added, for example, the nitrogen source is not added at the time of the configuration of the culture medium, so that only the microorganisms with the nitrogen fixation effect on the culture medium can survive!

The principle of Congo red medium:

The Congo red dye moves in the Congo red medium, and the Congo red dye enters the cellulase producing fungus. The cellulase producing fungus first decomposes the cellulose substance into the polysaccharide substance containing the structure of dextran and so on. The complex is not only adsorbed outside the hypanthium, but also can be further transported and absorbed into the hyphal. Through further degradation, the polysaccharide was decomposed and utilized, while the Congo red was retained in the hyphal, making the colony red. Therefore, cellulose-Congo red medium can be used as a specific medium for isolation and screening of cellulose decomposing fungi.

The material comes from the Internet.

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