What is the Good's buffer?

What is the Good's buffer?

Good's buffer is also known as amphoteric buffer. In 1960, N.E.Good and his colleagues summarized the advantages and disadvantages of various existing buffering reagents. Specific buffering systems dedicated to life science research must be designed and synthesized, and these buffers should have the following characteristics(http://www.hnhbsj.com):

1, the PKA value is between 6 and 8;

2, the solubility in water is high;

3. It is not easy to penetrate biofilm.

4, the salt effect is small;

5. Ion concentration, solution composition and temperature have little effect on dissociation.

6, do not form complexes or precipitates with metal ions;

7, the buffer is chemical stable;

8. the light absorption is small in the wavelength range of ultraviolet and visible light.

9, it is easy to make high purity salt.

Good's buffers are characterized by not participating in and not interfering with biochemical reactions, and have no inhibitory effect on enzyme chemical reactions, so they are specially used in the study of organelle and highly variable, pH-sensitive proteins and enzymes.

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