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Role of sodium pyruvate in cell culture
Sodium pyruvate is the most common pyruvate, and is a kind of endogenous small molecular substance, both of which are naturally present in human body and participate in the metabolism of whole body tissues and organs. Sodium pyruvate is widely used in medicine, diagnostic reagents, and medical devices as buffers, excipients and antioxidants. In addition, sodium pyruvate is one of the commonly used reagents in cell culture, what is the role of sodium pyruvate in cell culture?
The role of sodium keto in cell culture(http://www.hnhbsj.com):
in that daily life of the human body, sodium pyruvate is necessary for the activity of metabolic life, it is an energy substance, and in the cell culture test conducted in scientific research, the scientific research staff have also found a substance that has a similar effect, The substance that is similar to the energy of the body's metabolic activity that provides action to the human body, such as glucose, sodium lactate, etc., is sufficient for most of the cells in the human body to use only glucose, and sodium pyruvate is a necessary substance in cell culture. The sodium pyruvate functions as an alternative carbon source in the culture medium, and participates in the cell nutrition metabolism.
Preparation of sodium pyruvate:
Place the pyruvic acid in the workshop for more than one hour, wrap the clean polyester fiber cloth on the pipe opening, slowly insert it into the bottom of the bucket, vacuum the pyruvate 280kg into the sodium pyruvate reaction tank, pump it and stir it. The pretreated solution of 38 ~ 42 ℃ sodium carbonate was slowly pressed into the reaction tank (the control pressure was in 0.03~0.06MPa), and the temperature in the reaction tank was not more than 35 ℃ (if the temperature exceeded 35 ℃ during the reaction process, measures should be taken to reduce the temperature). The pH value should be measured during the reaction, when the pH value of the reaction solution reaches 4.8 × 5.3 (with precision test paper) When the feed is stopped, the feeding time is controlled for about 1 hour, the reaction is finished, and 1440kg 95% ethanol is slowly pumped into the reaction tank (coated with clean polyester fiber cloth at the end of the pipe) (the adding speed should be slow first and then fast. The starting 50% adding time shall be controlled within 60 minutes, and the last 50% shall be controlled within 30 minutes), after pumping, centrifugal drying, put into the special stainless steel barrel into the drying room, pour into the double cone vacuum drying oven, When the vacuum degree is ≥-0.09Mpa. the hot water temperature is 75 ~ 80 ℃, the internal temperature of the vacuum dryer is increased to 65 ℃, the drying time is recorded and the true value is controlled. The temperature in the air dryer is 65 / 70 ℃ for 2 hours. After drying for 2 hours, please check, dry loss ≤ 0.5%, cooling for about 20 minutes, output, sift with 20 mesh, package, into storage. The yield should be in the range of 105 ±5%.
The material comes from the Internet.
To learn more about the knowledge of biochemical reagents, please pay attention to the official website of the "Huibai reagent": http://www.hnhbsj.com
(All contents of this website, where the source is the "Huibai reagent", the copyright is owned by the Huibai reagent, is not authorized, any media, website or individual shall not be reproduced, otherwise the legal liability will be held, and the "Source: Huibai reagent" shall be indicated in the authorization and transfer. The source of this network is the content of other media. The content of the other media is reprinted. The copyright is only shared by the original author. If the copyright is infringed, please contact us in time. )
The role of sodium keto in cell culture(http://www.hnhbsj.com):
in that daily life of the human body, sodium pyruvate is necessary for the activity of metabolic life, it is an energy substance, and in the cell culture test conducted in scientific research, the scientific research staff have also found a substance that has a similar effect, The substance that is similar to the energy of the body's metabolic activity that provides action to the human body, such as glucose, sodium lactate, etc., is sufficient for most of the cells in the human body to use only glucose, and sodium pyruvate is a necessary substance in cell culture. The sodium pyruvate functions as an alternative carbon source in the culture medium, and participates in the cell nutrition metabolism.
Preparation of sodium pyruvate:
Place the pyruvic acid in the workshop for more than one hour, wrap the clean polyester fiber cloth on the pipe opening, slowly insert it into the bottom of the bucket, vacuum the pyruvate 280kg into the sodium pyruvate reaction tank, pump it and stir it. The pretreated solution of 38 ~ 42 ℃ sodium carbonate was slowly pressed into the reaction tank (the control pressure was in 0.03~0.06MPa), and the temperature in the reaction tank was not more than 35 ℃ (if the temperature exceeded 35 ℃ during the reaction process, measures should be taken to reduce the temperature). The pH value should be measured during the reaction, when the pH value of the reaction solution reaches 4.8 × 5.3 (with precision test paper) When the feed is stopped, the feeding time is controlled for about 1 hour, the reaction is finished, and 1440kg 95% ethanol is slowly pumped into the reaction tank (coated with clean polyester fiber cloth at the end of the pipe) (the adding speed should be slow first and then fast. The starting 50% adding time shall be controlled within 60 minutes, and the last 50% shall be controlled within 30 minutes), after pumping, centrifugal drying, put into the special stainless steel barrel into the drying room, pour into the double cone vacuum drying oven, When the vacuum degree is ≥-0.09Mpa. the hot water temperature is 75 ~ 80 ℃, the internal temperature of the vacuum dryer is increased to 65 ℃, the drying time is recorded and the true value is controlled. The temperature in the air dryer is 65 / 70 ℃ for 2 hours. After drying for 2 hours, please check, dry loss ≤ 0.5%, cooling for about 20 minutes, output, sift with 20 mesh, package, into storage. The yield should be in the range of 105 ±5%.
The material comes from the Internet.
To learn more about the knowledge of biochemical reagents, please pay attention to the official website of the "Huibai reagent": http://www.hnhbsj.com
(All contents of this website, where the source is the "Huibai reagent", the copyright is owned by the Huibai reagent, is not authorized, any media, website or individual shall not be reproduced, otherwise the legal liability will be held, and the "Source: Huibai reagent" shall be indicated in the authorization and transfer. The source of this network is the content of other media. The content of the other media is reprinted. The copyright is only shared by the original author. If the copyright is infringed, please contact us in time. )
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