Preparation of medium

Culture medium refers to a nutrient matrix made up of different nutrients for the growth and reproduction of microorganisms, plants, or animals (tissues). Generally contains carbohydrates, nitrogen substances, inorganic salts (including trace elements), vitamins and water and other major substances. Medium is the basic material to provide cell nutrition and promote cell proliferation.

It is also the living environment of cell growth and reproduction. The type of culture medium is a lot, and the source of the prepared raw material can be divided into.

Natural medium, synthetic medium and semi-synthetic medium can be divided into solid medium, liquid medium and semi-solid medium according to physical condition. According to the function of the medium, it can be divided into basic medium, selective medium, enrichment medium, identification medium and so on. It can be divided into bacterial culture medium, actinomycetes culture medium, yeast culture medium, fungus culture medium and so on. After the culture medium has been arranged, it is usually necessary to test and regulate PH, and to sterilize it, usually using high temperature and filtration sterilization. The medium is rich in nutrients, easy to be polluted or metamorphosed, it should not be released for a long time after it is prepared, it is better to use it now.

Medium configuration procedure:
1, ingredients:
Add a small amount of water (distilled water, natural water) to the container according to the formula.

2, dissolution:
Starch dissolution: a small amount of cold water is blended into a paste. Heat to dissolve and stir while heating to prevent charring.]

3, tune PH:
Adjust the medium to the required value with 1N hydrochloric acid or 1N NaOH.

4, filtering:
Filter paper or cotton for filtering.

5, sub-assembly:
The general medium is placed in a triangular flask or in a test tube for sterilization.

6. Bandage:
After subpackaging, the tampon is filled and the tampon is wrapped in kraft paper to prevent the water from entering the tampon to wet when the tampon is sterilized.

7. Sterilization:
High pressure steam sterilization according to the temperature and pressure required in the formulation. If the temperature of sterilization is too high, the nutrients will be destroyed.

8. Storage:
The culture medium is placed at 30 & deg; C for one day, and the culture medium can be used without pollution. The paper is usually wrapped in a refrigerator at 2-8 & deg; C for standby.

The material comes from the network.
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