The principle and precautions of Poly-L-Lysine (Poly-L-Lysine)
Poly-lysine solution is a widely used adhesive for tissue slices and glass slides. The polycation molecules interact with anions in tissue sections to produce strong adhesion. The properties of the culture bottle surface are very important for cell culture, and the glycoprotein (negative) on the cell surface is easy to adsorb on the hydrophilic surface, so if the cell culture surface has a significant amount of positive charge, it can promote the cell adsorption. This is where the use of polylysine to optimize the surface of cell culture is important.
Applications:
Suitable for tissue, immunohistochemistry, frozen section, cell smear, in situ hybridization and other glass slide anti-strip treatment, to prevent tissue from falling during the operation of the experiment. It can also be used in cell culture to increase cell adhesion ability.
Solution preparation:
Dissolved in deionized water and prepared as mother liquor. Stored at -20 ℃.
Glass processing:
1. Dilution of Polysine solution with sterilizing water is generally used at a concentration of 0.01.
2. Immerse the slide in a dilute poly-lysine solution for 5 minutes. Note that increasing the time does not improve the envelope effect.
3. Dry at 60 ℃ for 1 hour or overnight at room temperature 18-26 ℃.
Note:
1. Each 100mL diluted polylysine solution to be coated with 40-90 glass slides, more than 90 sheets will affect its adhesion.
2. Previous slides must be kept clean. Clean with 70% ethanol solution containing 1% HCl if necessary.
3. The released poly-lysine solution should be stable at 2-8 ℃ for at least 3 months.
4. Filter out used diluents and discard them if turbidity or bacteria appear
5. Poly lysine concentrations can be higher and can be reused at least 20 times.
Culture bottle handling:
1. Coated with a culture plate or a culture bottle, usually with a concentration of 0. 01 poly-lysine,
2. Prepare 100ml water and sterilize under high pressure.
3.0.01% poly-lysine was evenly coated on the surface of the culture substrate at a volume of 50 microliters per square centimeter, and remained at room temperature for 5 minutes to remove the excess liquid.
4. Add sterilizing water, wash repeatedly three times, sterile operation.
5. Dry and dry after treatment.
6. Polysine, which remains sterile after recovery, can be reused.
The material comes from the network.
For more information on biochemical reagents, check out Huibai Reagent's official website: http://www.hnhbsj.com
(All contents of this website, if the source is the “Huibai reagent”, the copyright is owned by the foreign exchange and all the reagents, without authorization, any media, website or individual shall not be reproduced, otherwise the legal liability will be held, and the “Source: Huibai reagent” shall be noted in the authorization and transfer. "The source of this network is the content of other media. The content of the other media is reproduced and reproduced only. The copyright belongs to the original author. If there is copyright infringement, please contact us in a timely manner.)
Applications:
Suitable for tissue, immunohistochemistry, frozen section, cell smear, in situ hybridization and other glass slide anti-strip treatment, to prevent tissue from falling during the operation of the experiment. It can also be used in cell culture to increase cell adhesion ability.
Solution preparation:
Dissolved in deionized water and prepared as mother liquor. Stored at -20 ℃.
Glass processing:
1. Dilution of Polysine solution with sterilizing water is generally used at a concentration of 0.01.
2. Immerse the slide in a dilute poly-lysine solution for 5 minutes. Note that increasing the time does not improve the envelope effect.
3. Dry at 60 ℃ for 1 hour or overnight at room temperature 18-26 ℃.
Note:
1. Each 100mL diluted polylysine solution to be coated with 40-90 glass slides, more than 90 sheets will affect its adhesion.
2. Previous slides must be kept clean. Clean with 70% ethanol solution containing 1% HCl if necessary.
3. The released poly-lysine solution should be stable at 2-8 ℃ for at least 3 months.
4. Filter out used diluents and discard them if turbidity or bacteria appear
5. Poly lysine concentrations can be higher and can be reused at least 20 times.
Culture bottle handling:
1. Coated with a culture plate or a culture bottle, usually with a concentration of 0. 01 poly-lysine,
2. Prepare 100ml water and sterilize under high pressure.
3.0.01% poly-lysine was evenly coated on the surface of the culture substrate at a volume of 50 microliters per square centimeter, and remained at room temperature for 5 minutes to remove the excess liquid.
4. Add sterilizing water, wash repeatedly three times, sterile operation.
5. Dry and dry after treatment.
6. Polysine, which remains sterile after recovery, can be reused.
The material comes from the network.
For more information on biochemical reagents, check out Huibai Reagent's official website: http://www.hnhbsj.com
(All contents of this website, if the source is the “Huibai reagent”, the copyright is owned by the foreign exchange and all the reagents, without authorization, any media, website or individual shall not be reproduced, otherwise the legal liability will be held, and the “Source: Huibai reagent” shall be noted in the authorization and transfer. "The source of this network is the content of other media. The content of the other media is reproduced and reproduced only. The copyright belongs to the original author. If there is copyright infringement, please contact us in a timely manner.)
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