The steps of Masson trichrome dyeing solution

Connective tissue in a narrow sense refers to the three fibers it contains: collagen fiber, reticular fiber, elastic fiber, and collagen fiber (collagenfiber) is the most widely distributed, the largest content of a fiber. Masson trichrome staining, also known as Ma Song staining, It is the most classical method of connective tissue staining and the authoritative and classical technique of collagen staining. The so-called trichrome staining usually refers to the staining of the nucleus and the selective display of collagen fibers and muscle fibers.

Operational steps (for reference only)Huibai Reagent's official website: http://www.hnhbsj.com:

1.Slice conventional dewaxing to water.

2,The prepared Weigert iron hematoxylin was dyed for 5 min.

3,Acid ethanol was used to differentiate and wash.

4.Return to blue with Masson blue solution, wash.

5.Wash distilled water for 1 min.

6.Lichun red fuchsin was dyed for 5 ~ 10 min.

7.In the above operation, the weak acid working liquid was prepared according to the ratio of distilled water: weak acid solution = 2:1, and washed with weak acid working liquid for 1 min.

8.The phosphomolybdic acid solution was washed for 1 ~ 2 min.

9.Wash for 1 min with the prepared weak acid working fluid.

10,It was dyed directly into aniline blue dye solution for 1 ~ 2 min.

11.Wash for 1 min with the prepared weak acid working fluid.

12.Rapid dehydration of 12% 95% ethanol.

13.Anhydrous ethanol was dehydrated for 3 times, each time for 5g / 10s.

14.Xylene was transparent for 3 times, one minute and two minutes each time.

15, neutral gum is sealed.

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