The steps of Masson trichrome dyeing solution
Connective tissue in a narrow sense refers to the three fibers it contains: collagen fiber, reticular fiber, elastic fiber, and collagen fiber (collagenfiber) is the most widely distributed, the largest content of a fiber. Masson trichrome staining, also known as Ma Song staining, It is the most classical method of connective tissue staining and the authoritative and classical technique of collagen staining. The so-called trichrome staining usually refers to the staining of the nucleus and the selective display of collagen fibers and muscle fibers.
Operational steps (for reference only)Huibai Reagent's official website: http://www.hnhbsj.com:
1.Slice conventional dewaxing to water.
2,The prepared Weigert iron hematoxylin was dyed for 5 min.
3,Acid ethanol was used to differentiate and wash.
4.Return to blue with Masson blue solution, wash.
5.Wash distilled water for 1 min.
6.Lichun red fuchsin was dyed for 5 ~ 10 min.
7.In the above operation, the weak acid working liquid was prepared according to the ratio of distilled water: weak acid solution = 2:1, and washed with weak acid working liquid for 1 min.
8.The phosphomolybdic acid solution was washed for 1 ~ 2 min.
9.Wash for 1 min with the prepared weak acid working fluid.
10,It was dyed directly into aniline blue dye solution for 1 ~ 2 min.
11.Wash for 1 min with the prepared weak acid working fluid.
12.Rapid dehydration of 12% 95% ethanol.
13.Anhydrous ethanol was dehydrated for 3 times, each time for 5g / 10s.
14.Xylene was transparent for 3 times, one minute and two minutes each time.
15, neutral gum is sealed.
The material comes from the network.
For more information on biochemical reagents, check out Huibai Reagent's official website: http://www.hnhbsj.com
(all contents of this website are marked as "Huibai Reagent", copyright is owned by Huibai Reagent. Any media, website or individual shall not be reproduced without authorization, otherwise, legal liability will be investigated. Authorization to reprint should be marked "source: Huibai Reagent". This website indicates the source is other media content is reprinted, reprint only for the viewpoint sharing, the copyright belongs to the original author all, if has the infringement copyright, please contact us in time.
Operational steps (for reference only)Huibai Reagent's official website: http://www.hnhbsj.com:
1.Slice conventional dewaxing to water.
2,The prepared Weigert iron hematoxylin was dyed for 5 min.
3,Acid ethanol was used to differentiate and wash.
4.Return to blue with Masson blue solution, wash.
5.Wash distilled water for 1 min.
6.Lichun red fuchsin was dyed for 5 ~ 10 min.
7.In the above operation, the weak acid working liquid was prepared according to the ratio of distilled water: weak acid solution = 2:1, and washed with weak acid working liquid for 1 min.
8.The phosphomolybdic acid solution was washed for 1 ~ 2 min.
9.Wash for 1 min with the prepared weak acid working fluid.
10,It was dyed directly into aniline blue dye solution for 1 ~ 2 min.
11.Wash for 1 min with the prepared weak acid working fluid.
12.Rapid dehydration of 12% 95% ethanol.
13.Anhydrous ethanol was dehydrated for 3 times, each time for 5g / 10s.
14.Xylene was transparent for 3 times, one minute and two minutes each time.
15, neutral gum is sealed.
The material comes from the network.
For more information on biochemical reagents, check out Huibai Reagent's official website: http://www.hnhbsj.com
(all contents of this website are marked as "Huibai Reagent", copyright is owned by Huibai Reagent. Any media, website or individual shall not be reproduced without authorization, otherwise, legal liability will be investigated. Authorization to reprint should be marked "source: Huibai Reagent". This website indicates the source is other media content is reprinted, reprint only for the viewpoint sharing, the copyright belongs to the original author all, if has the infringement copyright, please contact us in time.
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